protein

Recombinant Human HER4 / ErbB4 Protein (His & Fc tag)

MBS2546301

0.05 mg

405 USD

Recombinant Protein

Recombinant Human HER4 / ErbB4 Protein (His & Fc tag)

[HER4 / ErbB4]

[ALS19;HER4;p180erbB4]

[receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-2; Receptor tyrosine-protein kinase erbB-4; receptor tyrosine-protein kinase erbB-4; erb-b2 receptor tyrosine kinase 4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4]

[HER4 / ErbB4]

[ERBB4; ERBB4; HER4; ALS19; p180erbB4; HER4; 4ICD; E4ICD]

HEK293 Cells

> 95 % as determined by reducing SDS-PAGE.

Lyophilized from sterile PBS, pH 7.4, 5%trehalose, 5%mannitol, 0.01%Tween80.

Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

1. Measured by its ability to bind biotinylated human Fc-NRG1 (isoform Beta 1) in a functional ELISA. 2. Measured by its ability to bind with biotinylated human NRG1(isoform Beta 1)in a functional ELISA. 3. Measured by its ability to bind with biotinylated human NRG1 (aa 2-246)-Fc in a functional ELISA. 4. Measured by its ability to bind with biotinylated human NRG1 (aa 20-241)-His in a functional ELISA.

SDS-PAGE

Species: Human. Sequence: Met 1-Pro 651. Tag: C-His & Fc. Endotoxin: < 1.0 EU per ug as determined by the LAL method. Reconstitution: It is recommended that sterile water(200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV Vis. Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

110825958

NP_001036064.1

NM_001042599.1

Q15303

Mol_Mass: 97.5 kDa. AP_Mol_Mass: 125-135 kDa

Adaptive Immune System Pathway (366160); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downregulation Of ERBB4 Signaling Pathway (530745); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signal Transduction Pathway (106385)

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported. Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: basolateral plasma membrane; cytosol; extracellular region; mitochondrial matrix; mitochondrion; nucleoplasm; nucleus; plasma membrane; receptor complex. Molecular Function: epidermal growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor protein tyrosine kinase activity. Biological Process: cell migration; cell proliferation; central nervous system morphogenesis; embryonic pattern specification; heart development; lactation; MAPKKK cascade; mitochondrial fragmentation during apoptosis; negative regulation of cell proliferation; nervous system development; neural crest cell migration; olfactory bulb interneuron differentiation; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; positive regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid autophosphorylation; regulation of cell migration; regulation of phosphoinositide 3-kinase cascade; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Amyotrophic Lateral Sclerosis 19

0.05 mg

405 USD

0.1 mg

635