protein

Recombinant Human LRAP / ERAP2 Protein (His tag)

MBS2546109

0.01 mg

405 USD

Recombinant Protein

Recombinant Human LRAP / ERAP2 Protein (His tag)

[LRAP / ERAP2]

[FLJ23633, FLJ23701, FLJ23807, L-RAP, LRAP.ERAP2]

[endoplasmic reticulum aminopeptidase 2 isoform 1; Endoplasmic reticulum aminopeptidase 2; endoplasmic reticulum aminopeptidase 2; endoplasmic reticulum aminopeptidase 2; Leukocyte-derived arginine aminopeptidase; L-RAP]

[LRAP / ERAP2]

[ERAP2; ERAP2; LRAP; L-RAP; LRAP; L-RAP]

Human Cells

960

> 98 % as determined by SDS-PAGE

Lyophilized from sterile 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycercol

Samples are stable for up to twelve months from date of receipt at -70 degree C

The recombinant human ERAP2 consists of 921 amino acids and predictes a molecular mass of 106 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 115-125 kDa due to glycosylation.

Predicted N Terminal: Leu 24

Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method

Background: Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 960-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH (X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors. Description: A DNA sequence encoding the lumenal domain of human ERAP2 (NP_071745.1) (Ala 56-Thr 960) was expressed with a polyhistidine tag at the N-terminus.

11641261

NP_071745.1

NM_022350.4

Q6P179

40,088 Da

Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386)

This gene encodes a zinc metalloaminopeptidase of the M1 protease family that resides in the endoplasmic reticulum and functions in N-terminal trimming antigenic epitopes for presentation by major histocompatibility complex (MHC) class I molecules. Certain mutations in this gene are associated with the inflammatory arthritis syndrome ankylosing spondylitis and pre-eclampsia. This gene is located adjacent to a closely related aminopeptidase gene on chromosome 5. [provided by RefSeq, Jul 2016]

ERAP2: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys. Belongs to the peptidase M1 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.11.-; Endoplasmic reticulum; Membrane protein, integral; Protease. Chromosomal Location of Human Ortholog: 5q15. Cellular Component: endoplasmic reticulum lumen; plasma membrane. Molecular Function: aminopeptidase activity; endopeptidase activity; metallopeptidase activity; peptide binding; zinc ion binding. Biological Process: antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of peptide antigen via MHC class I; peptide catabolic process; regulation of blood pressure

0.01 mg

405 USD

0.02 mg

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