Recombinant Human CD96 Protein (His tag) | MyBioSource MBS2545870 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human CD96 Protein (His tag)

catalog :

MBS2545870

quantity :

0.02 mg

price :

365 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2545870

products type :

Recombinant Protein

products full name :

Recombinant Human CD96 Protein (His tag)

products short name :

[CD96]

products name syn :

[CD96]

other names :

[T-cell surface protein tactile isoform 2; T-cell surface protein tactile; T-cell surface protein tactile; CD96 molecule; Cell surface antigen CD96; T cell-activated increased late expression protein; CD_antigen: CD96]

products gene name :

[CD96]

other gene names :

[CD96; CD96; TACTILE]

sequence length :

569

purity :

> 90 % as determined by SDS-PAGE

form :

Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01%Tween80

storage stability :

Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles. Stability: Samples are stable for up to twelve months from date of receipt at -70°C

image1 heading :

SDS-PAGE

other info1 :

Source: Human. Expression Host: Human Cells. Protein Construction: A DNA sequence encoding the human CD96 isoform 2 (P40200-2) extracellular domain (Met 1-Met 503) was expressed, with a polyhistidine tag at the C-terminus. Predicted N Terminal: Val 22. Molecular Mass: The recombinant human CD96 consists of 493 amino acids and predictes a molecular mass of 55 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD96 is approximately 120-130 kDa due to high glycosylation.

other info2 :

Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method. Reconstitution: It is recommended that sterile water (200ul) be added to the vial to prepare a stock solution of 0.25mg/ml. Concentration is measured by UV-Vis.

products description :

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD155 ligand CD96 is a member of the Ig superfamily. It's a immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR), a nectins and nectin-like protein family member serve to mediate cell-cell adhesion, cell migration, with the presence of an additional receptor, CD96. CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. The effect the cells with mutated CD96 protein lost adhesion and growth activities indicates that CD96 mutations may cause a form of the C syndrome by interfering with cell adhesion and growth.

ncbi gi num :

5032141

ncbi acc num :

NP_005807.1

ncbi gb acc num :

NM_005816.4

uniprot acc num :

P40200

ncbi pathways :

Adaptive Immune System Pathway (366160); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

ncbi summary :

The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing generates multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]

uniprot summary :

CD96: May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Defects in CD96 are a cause of C syndrome (CSYN); also called Opitz trigonocephaly syndrome. This syndrome is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q13.13-q13.2. Cellular Component: adherens junction; cytoplasm; integral to plasma membrane; plasma membrane. Biological Process: cell adhesion; cell-matrix adhesion; immune response; regulation of immune response. Disease: C Syndrome

size1 :

0.02 mg

price1 :

365 USD

size2 :

0.05 mg

price2 :

735

more info or order :

MyBioSource product webpage