protein

Recombinant Human DMBT1 / Muclin Protein (His tag)

MBS2545695

0.05 mg

435 USD

Recombinant Protein

Recombinant Human DMBT1 / Muclin Protein (His tag)

[DMBT1 / Muclin]

[GP340;muclin]

[deleted in malignant brain tumors 1 protein isoform a; Deleted in malignant brain tumors 1 protein; deleted in malignant brain tumors 1 protein; deleted in malignant brain tumors 1; Glycoprotein 340; Gp-340; Hensin; Salivary agglutinin; SAG; Surfactant pulmonary-associated D-binding protein]

[DMBT1]

[DMBT1; DMBT1; SAG; GP340; SALSA; muclin; GP340; Gp-340; SAG]

HEK293 Cells

Met 1-Ser 220

> 95 % as determined by reducing SDS-PAGE.

Lyophilized from sterile PBS, pH 7.4, 5%trehalose, 5% mannitol, 0.01% Tween80.

Samples are stable for up to twelve months from date of receipt at -70 degree C.

The recombinant human DMBT1 consists of 211 amino acids and has a predicted molecular mass of 22.6 kDa. The apparent molecular mass of rh DMBT1 is approximately 35-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.

SDS-PAGE

Species: Human

Tag: C-His. Bio activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized recombinant human Galectin-3 at 10 ug/ml (100 ul/well) can bind biotinylated DMBT1-His with a linear range of 0.06-1.0 ug/ml. Endotoxin: < 1.0 EU per ?g as determined by the LAL method. Shipping note: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Reconstitution: It is recommended that sterile water(200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV- Vis.

Background: Deleted in malignant brain tumors 1 protein, also known as glycoprotein 340, surfactant pulmonary-associated D-binding protein, DMBT1 and GP340, is a secreted protein which belongs to the DMBT1 family. DMBT1 contains 2 CUB domains, 14 SRCR domains and 1 ZP domain. It is highly expressed in alveolar and macrophage tissues. In some macrophages, expression is detected on the membrane, and in other macrophages, it is strongly expressed in the phagosome/phagolysosome compartments. Defects in DMBT1 are involved in the development of glioma (GLM). Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Description: A DNA sequence encoding the N-terminal segment of human DMBT1 (NP_004397.2) (Met 1-Ser 220) was expressed, fused with a polyhistidine tag at the C-terminus.

148539840

NP_004397.2

NM_004406.2

Q9UGM3

Mol. Mass: 22.6 kDa. AP Mol .Mass: 35-45 kDa

Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

Loss of sequences from human chromosome 10q has been associated with the progression of human cancers. This gene was originally isolated based on its deletion in a medulloblastoma cell line. This gene is expressed with transcripts of 6.0, 7.5, and 8.0 kb in fetal lung and with one transcript of 8.0 kb in adult lung, although the 7.5 kb transcript has not been characterized. The encoded protein precursor is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR-interspersed domains (SID). Transcript variant 2 (8.0 kb) has been shown to bind surfactant protein D independently of carbohydrate recognition. This indicates that DMBT1 may not be a classical tumor suppressor gene, but rather play a role in the interaction of tumor cells and the immune system. [provided by RefSeq, Mar 2016]

DMBT1: May be considered as a candidate tumor suppressor gene for brain, lung, esophageal, gastric, and colorectal cancers. May play roles in mucosal defense system, cellular immune defense and epithelial differentiation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. May function as a binding protein in saliva for the regulation of taste sensation. Binds to HIV-1 envelope protein and has been shown to both inhibit and facilitate viral transmission. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly- sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell. Defects in DMBT1 are involved in the development of glioma (GLM). Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Homozygous deletions may be the predominant mechanism of DMBT1 inactivation playing a role in carcinogenesis. DMBT1 is deleted in medulloblastoma and glioblastoma cell lines; point mutations have also been reported in patients with glioma. A loss or reduction of DMBT1 expression has been seen in esophageal, gastric, lung and colorectal carcinomas as well. Belongs to the DMBT1 family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Oncoprotein; Secreted; Secreted, signal peptide; Tumor suppressor. Chromosomal Location of Human Ortholog: 10q26.13. Cellular Component: cytoplasm; extracellular region; extracellular space; extrinsic to membrane; phagocytic vesicle membrane; zymogen granule membrane. Molecular Function: calcium-dependent protein binding; pattern recognition receptor activity; protein binding; scavenger receptor activity. Biological Process: cellular protein metabolic process; epithelial cell differentiation; induction of bacterial agglutination; innate immune response. Disease: Glioma Susceptibility 1; Medulloblastoma

0.05 mg

435 USD

0.1 mg

735