Recombinant Human BCHE / Butyrylcholinesterase Protein (His tag) | MyBioSource MBS2545601 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human BCHE / Butyrylcholinesterase Protein (His tag)

catalog :

MBS2545601

quantity :

0.01 mg

price :

405 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2545601

products type :

Recombinant Protein

products full name :

Recombinant Human BCHE / Butyrylcholinesterase Protein (His tag)

products short name :

[BCHE / Butyrylcholinesterase]

products name syn :

[CHE1;CHE2;E1]

other names :

[cholinesterase; Cholinesterase; cholinesterase; butyrylcholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase]

products gene name :

[BCHE]

other gene names :

[BCHE; BCHE; E1; CHE1; CHE2; CHE1]

host :

HEK293 Cells

sequence positions :

[Met 1-Leu 602]

purity :

> 97 % as determined by reducing SDS-PAGE.

form :

Lyophilized from sterile 25mM Tris, 150mM NaCl, pH7.0, 5% Trehalose, 5% Mannitol, 0.01%Tween-80.

storage stability :

Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months. Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

app notes :

The recombinant human BCHE consists of 585 amino acids and has a predicted molecular mass of 66.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh BCHE is approximately 85-95 kDa due to glycosylation.

image1 heading :

SDS-PAGE

other info1 :

Species: Human. Tag: C-His. Bio activity: Measured by its ability to cleave Butyrylthiocholine. The specific activity is > 50,000 pmoles/min/ug.

other info2 :

Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method. Reconstitution: It is recommended that sterile water(80ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.

products description :

Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as "pseudo" or "nonneuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.

ncbi gi num :

4557351

ncbi acc num :

NP_000046.1

ncbi gb acc num :

NM_000055.3

ncbi mol weight :

66.5 kDa

ncbi pathways :

Glycerophospholipid Biosynthesis Pathway (645313); Irinotecan Pathway (198905); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Proteins Pathway (106230); Neuronal System Pathway (106513); Neurotransmitter Clearance In The Synaptic Cleft Pathway (106526); Peptide Hormone Metabolism Pathway (771603); Phospholipid Metabolism Pathway (645312); Synthesis Of PC Pathway (645319)

ncbi summary :

This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. [provided by RefSeq, Jul 2016]

uniprot summary :

BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family. Protein type: EC 3.1.1.8; Hydrolase; Nuclear envelope; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q26.1. Cellular Component: extracellular region. Molecular Function: acetylcholinesterase activity; cholinesterase activity; hydrolase activity, acting on ester bonds; identical protein binding. Biological Process: cocaine metabolic process

size1 :

0.01 mg

price1 :

405 USD

size2 :

0.02 mg

price2 :

635

more info or order :

MyBioSource product webpage