other

Acetylcholinesterase assay kit (Colorimetric method)

MBS2540435

50 Assays

250 USD

Assay Kit

Acetylcholinesterase assay kit (Colorimetric method)

[Acetylcholinesterase]

[acetylcholinesterase, partial; Acetylcholinesterase; acetylcholinesterase; acetylcholinesterase (Cartwright blood group)]

[ACHE; ACHE; YT; ACEE; ARACHE; N-ACHE; AChE]

ACES_HUMAN

72

This product has an approximately 2-2.5 month shelf life upon receipt. Please refer to the expiration date on the kit box.

Samples: Serum, Plasma, Or Tissue Samples.

Principle of the Assay: Acetylcholine Assay Kit measures the acetylcholine present within serum, plasma, or tissue samples. The assay is based on the enzyme driven reaction that will detect acetylcholine via acetylcholinesterase enzyme and choline oxidase. First, acetylcholinesterase hydrolyzes acetylcholine into choline and acetic acid. Choline is then oxidized by choline oxidase to produce hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorescence probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of acetylcholine standard within the 96-well microtiter plate format. Samples and standards are incubated for 45 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1)!!Background/Introduction: Acetylcholine is a polyatomic cation neurotransmitter that is produced in acetylcholinergic neurons. It is one of many neurotransmitters within the autonomic nervous system and the only neurotransmitter in the motor function of the somatic nervous system. Acetylcholine works within the peripheral and central nervous systems within many organisms, including humans. It is manufactured via choline acetyltransferase from acetyl-CoA and choline. Choline is an amine that is an essential nutrient that is a key precursor to many phospholipids. Acetylcholine works in the peripheral nervous system by activating skeletal muscles as well as smooth muscle and cardiac muscle function. Within the central nervous system, acetylcholine acts as a neuromodulator for the cholinergic system, which causes excitatory actions. Here the neurotransmitter is involved with plasticity, excitability, arousal, and reward. Acetylcholine's half-life and activity are very short because it is broken down by acetylcholinesterase. There are two main acetylcholine receptors: nicotinic and muscarinic. Acetylcholine disorders can have a profound impact on neurological function. A shortage of acetylcholine, such as the autoimmune disorder Myasthenia gravis, leads to muscle fatigue and weakness due to antibodies blocking acetylcholine receptors. Acetylcholine has also been implicated in many disease states including diabetic vasculopathy, hypertension, and Alzheimer's disease. Acetylcholine Assay Kit is a simple fluorometric assay that measures the amount of acetylcholine present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples. Sample acetylcholine concentrations are determined by comparison with a known acetylcholine standard. The kit's detection sensitivity limit is approximately 0.05 uM acetylcholine.

545225

58,352 Da

ATF-2 Transcription Factor Network Pathway (138006); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Biosynthesis Pathway (1270054); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Integrated Pancreatic Cancer Pathway (711360); Melatonin Metabolism And Effects Pathway (1458209); Metabolism Pathway (1269956)

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

AChE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.1.7; Hydrolase; Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; perinuclear region of cytoplasm; plasma membrane; synapse. Molecular Function: acetylcholine binding; acetylcholinesterase activity; beta-amyloid binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; protein binding; protein homodimerization activity; serine hydrolase activity. Biological Process: acetylcholine catabolic process; amyloid precursor protein metabolic process; cell adhesion; cell proliferation; DNA replication; muscle development; negative regulation of synaptic transmission, cholinergic; nervous system development; neurotransmitter biosynthetic process; osteoblast development; phosphatidylcholine biosynthetic process; positive regulation of protein secretion; response to wounding; synaptogenesis. Disease: Yt Blood Group Antigen

50 Assays

250 USD