Human SIRT3 (NAD-Dependent Deacetylase Sirtuin-3, Mitochondrial) ELISA Kit | MyBioSource MBS2540335 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human SIRT3 (NAD-Dependent Deacetylase Sirtuin-3, Mitochondrial) ELISA Kit

catalog :

MBS2540335

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2540335

products type :

ELISA Kit

products full name :

Human SIRT3 (NAD-Dependent Deacetylase Sirtuin-3, Mitochondrial) ELISA Kit

products short name :

[SIRT3 (NAD-Dependent Deacetylase Sirtuin-3, Mitochondrial)]

other names :

[NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform b; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sirtuin 3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3]

products gene name :

[SIRT3]

other gene names :

[SIRT3; SIRT3; SIR2L3; SIR2L3; hSIRT3]

uniprot entry name :

SIR3_HUMAN

reactivity :

Human

sequence length :

257

specificity :

This kit recognizes natural and some recombinant Human SIRT3. No Significant cross -reactivity or interference between HumanSIRT3and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.09ng/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SIRT3 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SIRT3. You can calculate the concentration of Human SIRT3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

63054862

ncbi acc num :

NP_001017524.1

ncbi gb acc num :

NM_001017524.2

ncbi mol weight :

28,567 Da

ncbi pathways :

Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Energy Metabolism Pathway (198907); Mitochondrial Biogenesis Pathway (1268839); Organelle Biogenesis And Maintenance Pathway (1268838); Signaling Events Mediated By HDAC Class I Pathway (137997); Signaling Events Mediated By HDAC Class III Pathway (137972); Transcriptional Activation Of Mitochondrial Biogenesis Pathway (1268841)

ncbi summary :

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product. Protein type: Deacetylase; EC 3.5.1.-; Mitochondrial. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: mitochondrial matrix; mitochondrion. Molecular Function: NAD+ ADP-ribosyltransferase activity; protein binding; zinc ion binding. Biological Process: aerobic respiration; mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage