Human SRD5a2 (Steroid 5 Alpha Reductase 2) ELISA Kit | MyBioSource MBS2540324 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human SRD5a2 (Steroid 5 Alpha Reductase 2) ELISA Kit

catalog :

MBS2540324

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2540324

products type :

ELISA Kit

products full name :

Human SRD5a2 (Steroid 5 Alpha Reductase 2) ELISA Kit

products short name :

[SRD5a2 (Steroid 5 Alpha Reductase 2)]

other names :

[steroid 5-alpha-reductase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; steroid 5 alpha-reductase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2; Type II 5-alpha reductase]

products gene name :

[SRD5a2]

other gene names :

[SRD5A2; SRD5A2; S5AR 2]

uniprot entry name :

S5A2_HUMAN

reactivity :

Human

sequence length :

254

specificity :

This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between HumansRANKLand analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human sRANKL concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sRANKL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sRANKL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sRANKL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human sRANKL. You can calculate the concentration of Human sRANKL in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

338469

ncbi acc num :

AAA60586.1

ncbi mol weight :

28,393 Da

ncbi pathways :

Androgen Biosynthesis Pathway (1270050); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Metabolism Of Steroid Hormones Pathway (1270046); Prostate Cancer Pathway (83111); Prostate Cancer Pathway (523); Steroid Hormone Biosynthesis Pathway (82940); Steroid Hormone Biosynthesis Pathway (301); Allopregnanolone Biosynthesis Pathway (1108771); Allopregnanolone Biosynthesis Pathway (1108836)

ncbi summary :

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]

uniprot summary :

SRD5A2: Converts testosterone (T) into 5-alpha- dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH). A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. Belongs to the steroid 5-alpha reductase family. Protein type: Oxidoreductase; Membrane protein, integral; Membrane protein, multi-pass; EC 1.3.1.22; Lipid Metabolism - androgen and estrogen. Chromosomal Location of Human Ortholog: 2p23. Cellular Component: endoplasmic reticulum membrane. Molecular Function: 3-oxo-5-alpha-steroid 4-dehydrogenase activity; sterol 5-alpha reductase activity. Biological Process: androgen biosynthetic process; androgen metabolic process; cell-cell signaling; male gonad development. Disease: Pseudovaginal Perineoscrotal Hypospadias

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage