ELISA/assay

Human KEAP1 (Kelch-like ECH-associated protein 1)ELISA Kit

MBS2540311

24-Strip-Wells

215 USD

ELISA Kit

Human KEAP1 (Kelch-like ECH-associated protein 1)ELISA Kit

[KEAP1 (Kelch-like ECH-associated protein 1) ]

[kelch-like ECH-associated protein 1; Kelch-like ECH-associated protein 1; kelch-like ECH-associated protein 1; kelch like ECH associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19]

[KEAP1]

[KEAP1; KEAP1; INrf2; KLHL19; INRF2; KIAA0132; KLHL19; INrf2]

KEAP1_HUMAN

Human

624

This kit recognizes natural and recombinantHumanKEAP1. No significant cross-reactivity or interference between HumanKEAP1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanKEAP1concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KEAP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KEAP1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KEAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of KEAP1.You can calculate the concentration of KEAP1in the samples by comparing the OD of the samples to the standard curve.

22027642

NP_036421.2

NM_012289.3

69,666 Da

Adaptive Immune System Pathway (1269171); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Immune System Pathway (1269170); Keap1-Nrf2 Pathway (198777); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ETGE amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ETGE -containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane. Protein type: Motility/polarity/chemotaxis; Transcription regulation; Ubiquitin conjugating system; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: cytoplasm; cytosol; microtubule organizing center; midbody; nucleoplasm. Molecular Function: protein binding; transcription factor binding; ubiquitin-protein ligase activity. Biological Process: cytoplasmic sequestering of transcription factor; negative regulation of transcription factor activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590