antibody

EIF2AK1 Polyclonal Antibody

MBS2538144

0.02 mL

110 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry - paraffin section

Antibody

EIF2AK1 Polyclonal Antibody

[HRI]

[EIF2AK1; HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2]

[HRI]

Polyclonal

IgG

Rabbit

Human, Mouse, Monkey

Affinity Purification

PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB), Immunohistochemistry - Paraffin (IHC-p), ELISA

WB: 1:500-1:2000. IHC: 1:100-1:300. ELISA: 1:40000

Western Blot (WB)

Immunogen: Synthesized peptide derived from the C-terminal region of human HRI.

Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.

Q9BQI3

Calculated: 71kDa. Observed: 71kDa

0.02 mL

110 USD

0.06 mL

150

0.12 mL

225

0.2 mL

360