ELISA/assay

Rat WNT10B ( Wingless-Type MMTV Integration Site Family, Member 10B) ELISA Kit

MBS2533600

24-Strip-Wells

215 USD

ELISA Kit

Rat WNT10B ( Wingless-Type MMTV Integration Site Family, Member 10B) ELISA Kit

[Wingless-Type MMTV Integration Site Family, Member 10B]

[Wingless-type MMTV integration site family, member 10B; Protein Wnt-10b; protein Wnt-10b; wingless-type MMTV integration site family member 10B; Protein Wnt-12]

[WNT10B]

[WNT10B; WNT10B; SHFM6; WNT-12; WNT12]

WN10B_HUMAN

Rat

389

This kit recognizes Rat VE-Cadherin in samples. No significant cross-reactivity or interference between Rat VE-Cadherin and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VE-Cadherin were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VE-Cadherin were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat VE-Cadherin concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat VE-Cadherin. You can calculate the concentration of Rat VE-Cadherin in the samples by comparing the OD of the samples to the standard curve.

64654936

AAH96356.1

20,483 Da

Adipogenesis Pathway (198832); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (1269570); DNA Damage Response (only ATM Dependent) Pathway (198827); Developmental Biology Pathway (1270302); GPCR Ligand Binding Pathway (1269544); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix. Molecular Function: frizzled binding; receptor agonist activity. Biological Process: cell cycle arrest; cell fate commitment; chondrocyte differentiation; G2/M transition of mitotic cell cycle; lipid metabolic process; myoblast cell differentiation involved in skeletal muscle regeneration; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of anagen; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell proliferation; positive regulation of epithelial cell differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription factor activity; positive regulation of transcriptional preinitiation complex assembly; protein stabilization; regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of skeletal muscle development; sensory perception of taste; skeletal muscle fiber development; smoothened signaling pathway; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin. Disease: Split-hand/foot Malformation 6

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590