catalog number :
MBS2533570
products type :
ELISA Kit
products full name :
Rat LDHA (Lactate Dehydrogenase A) ELISA Kit
products short name :
[Lactate Dehydrogenase A]
other names :
[lactate dehydrogenase A, partial; L-lactate dehydrogenase A chain; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59]
products gene name :
[LDHA]
other gene names :
[LDHA; LDH-A; LDH-M]
uniprot entry name :
LDHA_HUMAN
specificity :
This kit recognizes natural and some recombinant Rat LDHA. No Significantcross -reactivity or interference between Rat LDHAand analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL . Sensitivity: Min: 0.188ng/mL ; Max: 20ng/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat LDHA concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LDHA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LDHAand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LDHA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LDHA.You can calculate the concentration of LDHAin the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAB20418.1
ncbi mol weight :
26,712 Da
uniprot summary :
LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase. Chromosomal Location of Human Ortholog: 11p15.4. Cellular Component: cell-cell adherens junction; cytosol; membrane; nucleus. Molecular Function: L-lactate dehydrogenase activity; protein binding. Biological Process: pyruvate metabolic process. Disease: Glycogen Storage Disease Xi
size5 :
10x96-Strip-Wells