catalog number :
MBS2533566
products type :
ELISA Kit
products full name :
Rat sE-selectin (soluble E-selectin) ELISA Kit
products short name :
[soluble E-selectin]
specificity :
This kit recognizes Rat SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Rat SDF-1/CXCL12and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Rat SDF-1/CXCL12were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Rat SDF-1/CXCL12were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat SDF-1/CXCL12. You can calculate the concentration of RatSDF-1/CXCL12in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells