Rat ASM (Acid Sphingomyelinase) ELISA Kit | MyBioSource MBS2533557 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat ASM (Acid Sphingomyelinase) ELISA Kit

catalog :

MBS2533557

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2533557

products type :

ELISA Kit

products full name :

Rat ASM (Acid Sphingomyelinase) ELISA Kit

products short name :

[Acid Sphingomyelinase]

other names :

[acid sphingomyelinase; Sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase 1; Acid sphingomyelinase; aSMase]

products gene name :

[ASM]

other gene names :

[SMPD1; SMPD1; ASM; NPD; ASMASE; ASM; aSMase]

uniprot entry name :

ASM_HUMAN

reactivity :

Rat

sequence length :

629

specificity :

This kit recognizes natural and recombinantRatASM. No significant cross-reactivity or interference between RatASM and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL . Sensitivity: Min: 0.469ng/mL ; Max: 50ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatASMconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ASM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ASMand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ASM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ASM.You can calculate the concentration of ASMin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

179095

ncbi acc num :

AAA58377.1

ncbi mol weight :

69,624 Da

ncbi pathways :

Ceramide Signaling Pathway (138023); FAS (CD95) Signaling Pathway (138081); Glycosphingolipid Metabolism Pathway (1270099); IL2 Signaling Events Mediated By PI3K Pathway (137938); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Ovarian Infertility Genes Pathway (198801)

ncbi summary :

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

uniprot summary :

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Phosphodiesterase; Lipid Metabolism - sphingolipid; EC 3.1.4.12. Chromosomal Location of Human Ortholog: 11p15.4-p15.1. Cellular Component: endosome; extracellular space; lamellar body; lysosomal lumen; lysosome; plasma membrane. Molecular Function: hydrolase activity, acting on glycosyl bonds; protein binding; sphingomyelin phosphodiesterase activity. Biological Process: ceramide biosynthetic process; glycosphingolipid metabolic process; negative regulation of MAP kinase activity; nervous system development; positive regulation of apoptosis; positive regulation of protein amino acid dephosphorylation; response to cocaine; response to drug; signal transduction; sphingolipid metabolic process; sphingomyelin catabolic process; sphingomyelin metabolic process. Disease: Niemann-pick Disease, Type A; Niemann-pick Disease, Type B

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590