ELISA/assay

Rat CBG (Corticosteroid Binding Globulin) ELISA Kit

MBS2533539

24-Strip-Wells

215 USD

ELISA Kit

Rat CBG (Corticosteroid Binding Globulin) ELISA Kit

[Corticosteroid Binding Globulin]

[corticosteroid-binding globulin; Corticosteroid-binding globulin; corticosteroid-binding globulin; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6; Serpin A6; Transcortin]

[CBG]

[SERPINA6; SERPINA6; CBG; CBG; CBG]

CBG_HUMAN

Rat

405

This kit recognizes Rat CAT in samples. No significant cross-reactivity or interference between Rat CAT and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: 9.375pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CAT were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CAT were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CAT concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat CAT. You can calculate the concentration of Rat CAT in the samples by comparing the OD of the samples to the standard curve.

73858564

NP_001747.2

NM_001756.3

45,141 Da

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]

SERPINA6: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency). CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. Belongs to the serpin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 14q32.1. Cellular Component: extracellular space. Molecular Function: serine-type endopeptidase inhibitor activity; steroid binding. Biological Process: glucocorticoid metabolic process; transport. Disease: Corticosteroid-binding Globulin Deficiency

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590