catalog number :
MBS2533529
products type :
ELISA Kit
products full name :
Mouse NEO (Neogenin) ELISA Kit
products short name :
[Neogenin]
other names :
[neogenin; Neogenin; neogenin; neogenin 1; Immunoglobulin superfamily DCC subclass member 2]
products gene name :
[NEO]
other gene names :
[NEO1; NEO1; NGN; IGDCC2; NTN1R2; IGDCC2; NGN]
uniprot entry name :
NEO1_HUMAN
specificity :
This kit recognizes Mouse MYC in samples. No significant cross-reactivity or interference between Mouse MYC and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MYC were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MYC were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse MYC concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MYC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MYC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MYC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse MYC. You can calculate the concentration of Mouse MYC in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAC51287.1
ncbi mol weight :
158,827 Da
ncbi pathways :
Axon Guidance Pathway (1270303); CDO In Myogenesis Pathway (1270336); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (1270302); Myogenesis Pathway (1270335); Netrin-1 Signaling Pathway (1270314)
ncbi summary :
This gene encodes a cell surface protein that is a member of the immunoglobulin superfamily. The encoded protein consists of four N-terminal immunoglobulin-like domains, six fibronectin type III domains, a transmembrane domain and a C-terminal internal domain that shares homology with the tumor suppressor candidate gene DCC. This protein may be involved in cell growth and differentiation and in cell-cell adhesion. Defects in this gene are associated with cell proliferation in certain cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
uniprot summary :
NEO1: May be involved as a regulatory protein in the transition of undifferentiated proliferating cells to their differentiated state. May also function as a cell adhesion molecule in a broad spectrum of embryonic and adult tissues. Belongs to the immunoglobulin superfamily. DCC family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 15q22.3-q23. Cellular Component: Golgi apparatus; integral to plasma membrane; nucleoplasm; plasma membrane. Molecular Function: cadherin binding; receptor activity. Biological Process: axon guidance; cell adhesion; iron ion homeostasis; muscle cell differentiation; myoblast fusion; negative regulation of protein secretion; positive regulation of BMP signaling pathway; positive regulation of muscle cell differentiation
size5 :
10x96-Strip-Wells