Mouse LBP (Lipopolysaccharide Binding Protein) ELISA Kit | MyBioSource MBS2533514 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse LBP (Lipopolysaccharide Binding Protein) ELISA Kit

catalog :

MBS2533514

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2533514

products type :

ELISA Kit

products full name :

Mouse LBP (Lipopolysaccharide Binding Protein) ELISA Kit

products short name :

[Lipopolysaccharide Binding Protein]

other names :

[lipopolysaccharide binding protein (LBP), partial; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; lipopolysaccharide binding protein]

products gene name :

[LBP]

other gene names :

[LBP; LBP; BPIFD2; LBP]

uniprot entry name :

LBP_HUMAN

reactivity :

Mouse

sequence length :

477

specificity :

This kit recognizes natural and some recombinant Mouse LBP. No significant crossreactivity or interference between Mouse LBP and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse LBP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse LBP. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse LBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse LBP, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse LBP. The concentration of Mouse LBP in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

186966

ncbi acc num :

AAA59493.1

ncbi mol weight :

53,384 Da

ncbi pathways :

IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); NF-kappa B Signaling Pathway (634527); Regulation Of Toll-like Receptor Signaling Pathway (920955); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (1269234); Toll-Like Receptors Cascades Pathway (1269204); Toll-like Receptor Signaling Pathway (83076)

ncbi summary :

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

uniprot summary :

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: cell surface; extracellular region; extracellular space; membrane. Molecular Function: lipopolysaccharide binding; protein binding; receptor binding. Biological Process: acute-phase response; cellular defense response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; detection of molecule of bacterial origin; innate immune response; leukocyte chemotaxis during inflammatory response; lipopolysaccharide transport; lipopolysaccharide-mediated signaling pathway; liver development; macrophage activation during immune response; negative regulation of tumor necrosis factor production; opsonization; positive regulation of chemokine production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of macrophage activation; positive regulation of phagocytosis, engulfment; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; response to lipopolysaccharide; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590