ELISA/assay

Mouse AP-1 (Transcription Factor AP-1) ELISA Kit

MBS2533495

24-Strip-Wells

215 USD

ELISA Kit

Mouse AP-1 (Transcription Factor AP-1) ELISA Kit

[Transcription Factor AP-1]

[transcription factor AP-1; Transcription factor AP-1; transcription factor AP-1; jun proto-oncogene; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39]

[AP-1]

[JUN; JUN; AP1; AP-1; c-Jun; AP1]

JUN_HUMAN

Mouse

331

This kit recognizes natural and recombinantMouseAP-1. No significant cross-reactivity or interference between MouseAP-1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL . Sensitivity: Min: 46.875pg/mL ; Max: 5000pg/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseAP-1concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AP-1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AP-1.You can calculate the concentration of AP-1in the samples by comparing the OD of the samples to the standard curve.

4758616

NP_002219.1

NM_002228.3

35,676 Da

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (1269236); Activation Of HOX Genes During Differentiation Pathway (1339139); Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway (1339140); Activation Of The AP-1 Family Of Transcription Factors Pathway (1269218); AhR Pathway (755436); Amphetamine Addiction Pathway (547607)

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590