Mouse Itgam/CD11b (Integrin alpha-M) ELISA Kit | MyBioSource MBS2533464 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Itgam/CD11b (Integrin alpha-M) ELISA Kit

catalog :

MBS2533464

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2533464

products type :

ELISA Kit

products full name :

Mouse Itgam/CD11b (Integrin alpha-M) ELISA Kit

products short name :

[Integrin alpha-M]

products name syn :

[CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6; antigen CD11b (p170); integrin alpha M; macrophage antigen alpha polypeptide; neutrophil adherence receptor alpha-M subunit]

other names :

[integrin alpha-M isoform 1; Integrin alpha-M; integrin alpha-M; integrin subunit alpha M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b]

products gene name :

[Itgam/CD11b]

other gene names :

[ITGAM; ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; CD11B; CR3A]

uniprot entry name :

ITAM_HUMAN

reactivity :

Mouse

sequence length :

1153

specificity :

This kit recognizes Mouse IL-6 in samples. No significant cross-reactivity or interference between Mouse IL-6 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-6 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-6 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse IL-6 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IL-6. You can calculate the concentration of Mouse IL-6 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

224831239

ncbi acc num :

NP_001139280.1

ncbi gb acc num :

NM_001145808.1

ncbi mol weight :

127,307 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extracellular Matrix Organization Pathway (1270244); Focal Adhesion Pathway (198795); Hematopoietic Cell Lineage Pathway (83078)

ncbi summary :

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: cell surface; extracellular space; plasma membrane. Molecular Function: glycoprotein binding; protein binding. Biological Process: extracellular matrix organization and biogenesis; leukocyte migration; toll-like receptor 4 signaling pathway. Disease: Systemic Lupus Erythematosus, Susceptibility To, 6

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590