Mouse HIF-2alpha (Hypoxia Inducible Factor 2 Alpha) ELISA Kit | MyBioSource MBS2533460 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse HIF-2alpha (Hypoxia Inducible Factor 2 Alpha) ELISA Kit

catalog :

MBS2533460

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2533460

products type :

ELISA Kit

products full name :

Mouse HIF-2alpha (Hypoxia Inducible Factor 2 Alpha) ELISA Kit

products short name :

[Hypoxia Inducible Factor 2 Alpha]

other names :

[hypoxia inducible factor 2 alpha; Endothelial PAS domain-containing protein 1; endothelial PAS domain-containing protein 1; endothelial PAS domain protein 1; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha]

products gene name :

[HIF-2alpha]

other gene names :

[Epas1; Epas1; HLF; Hif2a; Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha]

uniprot entry name :

EPAS1_RAT

reactivity :

Mouse

sequence length :

874

specificity :

This kit recognizes Mouse HIF-1alpha in samples. No Significant cross-reactivity or interference between Mouse HIF-1alpha and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 125.00-8000 pg/mL. Sensitivity: 75.00 pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse HIF-1alpha were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse HIF-1alpha were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse HIF-1alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-1alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse HIF-1alpha. You can calculate the concentration of Mouse HIF-1alpha in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

8953577

ncbi acc num :

CAB96612.1

uniprot acc num :

Q9JHS1

ncbi mol weight :

96,718 Da

ncbi pathways :

Adipogenesis Pathway (198479); Cellular Response To Hypoxia Pathway (1333253); Cellular Responses To Stress Pathway (1333252); Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (1333255); Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (1333256); Pathways In Cancer (83493); Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway (1333254); Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway (1333257); Renal Cell Carcinoma Pathway (83495); Renal Cell Carcinoma Pathway (519)

ncbi summary :

activates luciferase activity after transfection [RGD, Feb 2006]

uniprot summary :

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage