catalog number :
MBS2533439
products type :
ELISA Kit
products full name :
Human CoQ10B (Coenzyme Q10 Homolog B) ELISA Kit
products short name :
[CoQ10B (Coenzyme Q10 Homolog B)]
other names :
[coenzyme Q10 homolog A (yeast), isoform CRA_c, partial; Coenzyme Q-binding protein COQ10 homolog A, mitochondrial; coenzyme Q-binding protein COQ10 homolog A, mitochondrial; coenzyme Q10A]
products gene name :
[CoQ10B]
other gene names :
[COQ10A; COQ10A]
uniprot entry name :
CQ10A_HUMAN
specificity :
This kit recognizes Human COL18alpha1 in samples. No Significant cross-reactivity or interference between Human COL18alpha1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.10 ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human COL18alpha1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human COL18alpha1 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human COL18alpha1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL18alpha1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL18alpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL18alpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human COL18alpha1. You can calculate the concentration of Human COL18alpha1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
EAW96922.1
ncbi mol weight :
24,460 Da
uniprot summary :
COQ10A: Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes (Probable). Belongs to the COQ10 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: mitochondrial inner membrane
size5 :
10x96-Strip-Wells