Human CHIT1 (Chitotriosidase-1) ELISA Kit | MyBioSource MBS2533435 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CHIT1 (Chitotriosidase-1) ELISA Kit

catalog :

MBS2533435

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2533435

products type :

ELISA Kit

products full name :

Human CHIT1 (Chitotriosidase-1) ELISA Kit

products short name :

[Chitotriosidase-1]

other names :

[chitotriosidase-1 isoform 1; Chitotriosidase-1; chitotriosidase-1; chitinase 1; Chitinase-1]

products gene name :

[CHIT1]

other gene names :

[CHIT1; CHIT1; CHI3; CHIT; CHITD]

uniprot entry name :

CHIT1_HUMAN

reactivity :

Human

sequence length :

466

specificity :

This kit recognizes Human CGRP1 in samples. No Significant cross-reactivity or interference between Human CGRP1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000 pg/mL. Sensitivity: 9.38 pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CGRP1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CGRP1 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CGRP1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CGRP1. You can calculate the concentration of Human CGRP1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4502809

ncbi acc num :

NP_003456.1

ncbi gb acc num :

NM_003465.2

uniprot acc num :

Q13231

ncbi mol weight :

49,613 Da

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957)

ncbi summary :

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.2.1.14; Hydrolase; Secreted; Secreted, signal peptide; Carbohydrate Metabolism - amino sugar and nucleotide sugar. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: extracellular region; extracellular space; lysosome. Molecular Function: chitin binding; chitinase activity; endochitinase activity. Biological Process: carbohydrate metabolic process; chitin catabolic process; immune response; polysaccharide catabolic process; response to bacterium. Disease: Chitotriosidase Deficiency

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage