Human ADORA2a (Adenosine A2a Receptor) ELISA Kit | MyBioSource MBS2533431 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ADORA2a (Adenosine A2a Receptor) ELISA Kit

catalog :

MBS2533431

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2533431

products type :

ELISA Kit

products full name :

Human ADORA2a (Adenosine A2a Receptor) ELISA Kit

products short name :

[Adenosine A2a Receptor]

other names :

[adenosine A2a receptor; Adenosine receptor A2a; adenosine receptor A2a; adenosine A2a receptor]

products gene name :

[ADORA2a]

other gene names :

[ADORA2A; ADORA2A; A2aR; RDC8; ADORA2; ADORA2]

uniprot entry name :

AA2AR_HUMAN

reactivity :

Human

sequence length :

412

specificity :

This kit recognizes natural and recombinantHumanADORA2a. No significant cross-reactivity or interference between HumanADORA2a and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanADORA2aconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADORA2a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADORA2aand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADORA2a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADORA2a.You can calculate the concentration of ADORA2ain the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

119580062

ncbi acc num :

EAW59658.1

ncbi mol weight :

44,707 Da

ncbi pathways :

Activation Of TRKA Receptors Pathway (1269461); Adenosine P1 Receptors Pathway (1269565); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (s) Signalling Events Pathway (1269575); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544)

ncbi summary :

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

uniprot summary :

ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: integral to plasma membrane; membrane; plasma membrane. Molecular Function: adenosine receptor activity, G-protein coupled; enzyme binding; identical protein binding; protein binding. Biological Process: adenosine receptor signaling pathway; adenylate cyclase activation; apoptosis; blood circulation; blood coagulation; cAMP biosynthetic process; cell-cell signaling; cellular defense response; cellular protein metabolic process; central nervous system development; G-protein signaling, coupled to cAMP nucleotide second messenger; inflammatory response; nerve growth factor receptor signaling pathway; phagocytosis; sensory perception; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590