Human ATGL (Adipose Triglyceride Lipase) ELISA Kit | MyBioSource MBS2533410 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ATGL (Adipose Triglyceride Lipase) ELISA Kit

catalog :

MBS2533410

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2533410

products type :

ELISA Kit

products full name :

Human ATGL (Adipose Triglyceride Lipase) ELISA Kit

products short name :

[Adipose Triglyceride Lipase]

other names :

[adipose triglyceride lipase; Patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2; patatin like phospholipase domain containing 2; Adipose triglyceride lipase; Calcium-independent phospholipase A2; Desnutrin; IPLA2-zeta; Pigment epithelium-derived factor; TTS2.2; Transport-secretion protein 2; TTS2]

products gene name :

[ATGL]

other gene names :

[PNPLA2; PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik; ATGL; TTS2]

uniprot entry name :

PLPL2_HUMAN

reactivity :

Human

sequence length :

504

specificity :

This kit recognizes natural and recombinantHumanATGL. No significant cross-reactivity or interference between HumanATGL and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanATGLconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ATGL. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ATGLand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ATGL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ATGL.You can calculate the concentration of ATGLin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

58759051

ncbi acc num :

AAW81962.1

ncbi mol weight :

19,875 Da

ncbi pathways :

Acyl Chain Remodeling Of DAG And TAG Pathway (1270056); Acylglycerol Degradation Pathway (413388); Acylglycerol Degradation Pathway (468272); Fatty Acid Beta Oxidation Pathway (198865); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Glycerophospholipid Biosynthesis Pathway (1270054); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001)

ncbi summary :

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

uniprot summary :

PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Lipase; EC 3.1.1.3. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: cytosol; endoplasmic reticulum membrane; integral to membrane; lipid particle; plasma membrane. Molecular Function: triacylglycerol lipase activity. Biological Process: glycerophospholipid biosynthetic process; phospholipid metabolic process; sequestering of lipid; triacylglycerol catabolic process. Disease: Neutral Lipid Storage Disease With Myopathy

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage