catalog number :
MBS2533409
products type :
ELISA Kit
products full name :
Human FAAH (Fatty-acid amide hydrolase) ELISA kit
products short name :
[Fatty-acid amide hydrolase]
other names :
[fatty acid amide hydrolase; Fatty-acid amide hydrolase 1; fatty-acid amide hydrolase 1; fatty acid amide hydrolase; Anandamide amidohydrolase 1; Oleamide hydrolase 1]
products gene name :
[FAAH]
other gene names :
[FAAH; FAAH; PSAB; FAAH-1; FAAH1]
uniprot entry name :
FAAH1_HUMAN
specificity :
his kit recognizes Human F8 in samples. No Significant cross-reactivity or interference between HumanF8 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Otherbiological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 46.88-3000 mIU/mL. Sensitivity: 28.13 mIU/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human F8 concentrations in serum, plasma and otherbiological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated withan antibody specific to Human F8. Standards or samples are added to the micro ELISA plate wells and combined with thespecific antibody. Then a biotinylated detection antibody specific for Human F8 and Avidin-Horseradish Peroxidase(HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. Thesubstrate solution is added to each well. Only those wells that contain Human F8, biotinylated detection antibody andAvidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stopsolution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm +/- 2 nm. The OD value is proportional to the concentration of Human F8. You can calculate the concentration ofHuman F8 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAD13768.1
ncbi mol weight :
63,066 Da
ncbi pathways :
Arachidonic Acid Metabolism Pathway (1270087); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Retrograde Endocannabinoid Signaling Pathway (537443); Retrograde Endocannabinoid Signaling Pathway (539806); Anandamide Degradation Pathway (142420); Anandamide Degradation Pathway (139489)
ncbi summary :
This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]
uniprot summary :
FAAH: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. Homodimer. Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. Inhibited by O-aryl carbamates and alpha-keto heterocytes. Belongs to the amidase family. Protein type: EC 3.5.1.99; Hydrolase; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p35-p34. Cellular Component: cytoskeleton; endoplasmic reticulum membrane; integral to membrane. Molecular Function: acylglycerol lipase activity; carbon-nitrogen ligase activity, with glutamine as amido-N-donor; fatty acid amide hydrolase activity; protein binding. Biological Process: arachidonic acid metabolic process; fatty acid catabolic process. Disease: Polysubstance Abuse, Susceptibility To
size5 :
10x96-Strip-Wells