Human KRT1 (Keratin 1) ELISA Kit | MyBioSource MBS2533398 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human KRT1 (Keratin 1) ELISA Kit

catalog :

MBS2533398

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2533398

products type :

ELISA Kit

products full name :

Human KRT1 (Keratin 1) ELISA Kit

products short name :

[Keratin 1]

other names :

[keratin 1; Keratin, type II cytoskeletal 1; keratin, type II cytoskeletal 1; keratin 1, type II; 67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1]

products gene name :

[KRT1]

other gene names :

[KRT1; KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK; KRTA; CK-1; K1]

uniprot entry name :

K2C1_HUMAN

reactivity :

Human

sequence length :

644

specificity :

This kit recognizes natural and recombinantHumanKRT1. No significant cross-reactivity or interference between HumanKRT1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanKRT1concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KRT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KRT1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of KRT1.You can calculate the concentration of KRT1in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

186772

ncbi acc num :

AAB47721.1

ncbi mol weight :

66,039 Da

ncbi pathways :

Corticotropin-releasing Hormone Pathway (920957); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352)

ncbi summary :

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

uniprot summary :

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 12q13.13. Cellular Component: cytoskeleton; extracellular matrix; extracellular space; keratin filament; membrane; nucleus; plasma membrane. Molecular Function: carbohydrate binding; protein binding; receptor activity; structural molecule activity. Biological Process: complement activation, lectin pathway; fibrinolysis; negative regulation of inflammatory response; regulation of angiogenesis; response to oxidative stress; retinal homeostasis. Disease: Epidermolytic Hyperkeratosis; Ichthyosis Hystrix, Curth-macklin Type; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis; Keratosis Palmoplantaris Striata Iii; Palmoplantar Keratoderma, Epidermolytic; Palmoplantar Keratoderma, Nonepidermolytic

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590