Human CYP2A6 (Cytochrome P450 2A6) ELISA Kit | MyBioSource MBS2533380 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CYP2A6 (Cytochrome P450 2A6) ELISA Kit

catalog :

MBS2533380

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2533380

products type :

ELISA Kit

products full name :

Human CYP2A6 (Cytochrome P450 2A6) ELISA Kit

products short name :

[Cytochrome P450 2A6]

other names :

[cytochrome P450 2A6; Cytochrome P450 2A6; cytochrome P450 2A6; cytochrome P450 family 2 subfamily A member 6; 1,4-cineole 2-exo-monooxygenase; CYPIIA6; Coumarin 7-hydroxylase; Cytochrome P450 IIA3; Cytochrome P450(I)]

products gene name :

[CYP2A6]

other gene names :

[CYP2A6; CYP2A6; CPA6; CYP2A; CYP2A3; P450PB; CYPIIA6; P450C2A; CYP2A3]

uniprot entry name :

CP2A6_HUMAN

reactivity :

Human

sequence length :

494

specificity :

This kit recognizes Human CXCR2 in samples. No significant cross-reactivity or interference between HumanCXCR2and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.47ng/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CXCR2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CXCR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CXCR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CXCR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CXCR2. You can calculate the concentration of Human CXCR2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

189339233

ncbi acc num :

NP_000753.3

ncbi gb acc num :

NM_000762.5

ncbi mol weight :

56,501 Da

ncbi pathways :

Biological Oxidations Pathway (1270189); CYP2E1 Reactions Pathway (1270196); Caffeine Metabolism Pathway (82945); Caffeine Metabolism Pathway (308); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Cytochrome P450 - Arranged By Substrate Type Pathway (1270191); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Drug Metabolism - Other Enzymes Pathway (83033)

ncbi summary :

This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6. [provided by RefSeq, Jul 2008]

uniprot summary :

CYP2A6: Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4- cineole 2-exo-monooxygenase. Possesses low phenacetin O- deethylation activity. Belongs to the cytochrome P450 family. Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Secondary Metabolites Metabolism - caffeine; EC 1.14.14.1; Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: cytoplasmic microtubule; endoplasmic reticulum membrane. Molecular Function: arachidonic acid epoxygenase activity; coumarin 7-hydroxylase activity; enzyme binding; heme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; steroid hydroxylase activity. Biological Process: coumarin catabolic process; coumarin metabolic process; drug metabolic process; epoxygenase P450 pathway; exogenous drug catabolic process; steroid metabolic process; xenobiotic metabolic process. Disease: Coumarin Resistance; Lung Cancer; Tobacco Addiction, Susceptibility To

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590