Human ADAMTS12 (A Disintegrin And Metalloproteinase With Thrombospondin 12) ELISA Kit | MyBioSource MBS2533373 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ADAMTS12 (A Disintegrin And Metalloproteinase With Thrombospondin 12) ELISA Kit

catalog :

MBS2533373

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2533373

products type :

ELISA Kit

products full name :

Human ADAMTS12 (A Disintegrin And Metalloproteinase With Thrombospondin 12) ELISA Kit

products short name :

[A Disintegrin And Metalloproteinase With Thrombospondin 12]

other names :

[A disintegrin and metalloproteinase with thrombospondin motifs 12 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 12; A disintegrin and metalloproteinase with thrombospondin motifs 12; ADAM metallopeptidase with thrombospondin type 1 motif 12]

products gene name :

[ADAMTS12]

other gene names :

[ADAMTS12; ADAMTS12; PRO4389; ADAM-TS 12; ADAM-TS12; ADAMTS-12]

uniprot entry name :

ATS12_HUMAN

reactivity :

Human

sequence length :

1594

specificity :

This kit recognizes Human ADAM10 in samples. No significant cross-reactivity or interference between HumanADAM10 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 37.5pg/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ADAM10 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADAM10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAM10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAM10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-subst rate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ADAM10. You can calculate the concentration of Human ADAM10 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

51558724

ncbi acc num :

NP_112217.2

ncbi gb acc num :

NM_030955.2

ncbi mol weight :

168,460 Da

ncbi pathways :

Metabolism Of Proteins Pathway (1268677); O-glycosylation Of TSR Domain-containing Proteins Pathway (1268735); O-linked Glycosylation Pathway (1268734); Post-translational Protein Modification Pathway (1268701)

ncbi summary :

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS-1) motif. Individual members of this family differ in the number of C-terminal TS-1 motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains eight TS-1 motifs. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. [provided by RefSeq, Jul 2008]

uniprot summary :

ADAMTS12: Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.24.-; Protease; Secreted. Chromosomal Location of Human Ortholog: 5q35. Cellular Component: extracellular matrix; proteinaceous extracellular matrix. Molecular Function: metalloendopeptidase activity; protein binding; zinc ion binding. Biological Process: cell migration; cell-matrix adhesion; negative regulation of chondrocyte differentiation; proteoglycan catabolic process; proteolysis involved in cellular protein catabolic process; regulation of inflammatory response

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage