ELISA/assay

Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA kit

MBS2533349

24-Strip-Wells

215 USD

ELISA Kit

Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA kit

[HLA class II histocompatibility antigen gamma chain]

[HLA class II histocompatibility antigen gamma chain isoform a; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen gamma chain; CD74 molecule; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD_antigen: CD74]

[CD74]

[CD74; CD74; II; DHLAG; HLADG; Ia-GAMMA; DHLAG; Ii]

HG2A_HUMAN

Human

296

This kit recognizes natural and some recombinant Human CD74. No significant crossreactivity or interference between Human CD74 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human CD74 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CD74. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CD74 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CD74, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD74. The concentration of Human CD74 in samples can be calculated by comparing the OD of the samples with the standard curve.

68448544

NP_001020330.1

NM_001025159.2

18,328 Da

Adaptive Immune System Pathway (1269171); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Hemostasis Pathway (1269340); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Immune System Pathway (1269170); MHC Class II Antigen Presentation Pathway (1269200); Tuberculosis Pathway (213780)

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Endoplasmic reticulum; Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 5q32. Cellular Component: cell surface; Golgi membrane; integral to membrane; intracellular; lysosomal lumen; lysosomal membrane; membrane; MHC class II protein complex; plasma membrane; trans-Golgi network membrane; vacuole. Molecular Function: beta-amyloid binding; cytokine binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; MHC class II protein binding; MHC class II protein binding, via antigen binding groove; protein binding. Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cell proliferation; immunoglobulin mediated immune response; intracellular protein transport; leukocyte migration; negative regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of peptide secretion; positive regulation of B cell proliferation; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; prostaglandin biosynthetic process; protein complex assembly; signal transduction

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590