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Human S100B (S100 Calcium Binding Protein B) CLIA Kit

MBS2532844

48-Strip-Wells

450 USD

CLIA Kit

Human S100B (S100 Calcium Binding Protein B) CLIA Kit

[S100B (S100 Calcium Binding Protein B)]

[S100-B; NEF; S100Beta]

[S100 calcium binding protein B; Protein S100-B; protein S100-B; S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B]

[S100B]

[S100B; S100B; NEF; S100; S100-B; S100beta]

S100B_HUMAN

Human

92

This kit recognizes natural and recombinant S100B. No significant cross-reactivity or interference between S100B and analogues was observed.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of S100B concentrations in serum, plasma and other biological fluids. Principle of the Assay This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to S100B. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of S100B. You can calculate the concentration of S100B in the samples by comparing the RLU value of the samples to the standard curve.

12804681

AAH01766.1

10,713 Da

Activated TLR4 Signalling Pathway (1269236); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1269258); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); MyD88 Cascade Initiated On Plasma Membrane Pathway (1269207); MyD88 Dependent Cascade Initiated On Endosome Pathway (1269228); MyD88-independent TLR3/TLR4 Cascade Pathway (1269220); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (1269237)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus. Molecular Function: identical protein binding; protein binding; RAGE receptor binding; protein homodimerization activity; zinc ion binding; calcium ion binding; calcium-dependent protein binding; tau protein binding. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; central nervous system development; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; regulation of cell shape; cell proliferation; learning and/or memory; axonogenesis; astrocyte differentiation; positive regulation of cell proliferation; response to methylmercury; innate immune response; regulation of neuronal synaptic plasticity

48-Strip-Wells

450 USD

96-Strip-Wells

535

5x96-Strip-Wells

2255

10x96-Strip-Wells

3975