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Human HTRA1 (HtrA Serine Peptidase 1) CLIA Kit

MBS2531908

48-Strip-Wells

450 USD

CLIA Kit

Human HTRA1 (HtrA Serine Peptidase 1) CLIA Kit

[HTRA1 (HtrA Serine Peptidase 1)]

[ARMD7; CARASIL; HtrA; L56; ORF480; PRSS11]

[HtrA serine peptidase 1; Serine protease HTRA1; serine protease HTRA1; HtrA serine peptidase 1; High-temperature requirement A serine peptidase 1; L56; Serine protease 11]

[HTRA1]

[HTRA1; HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; HTRA; PRSS11]

HTRA1_HUMAN

Human

480

This kit recognizes natural and recombinant HTRA1. No significant cross-reactivity or interference between HTRA1 and analogues was observed.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of HTRA1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HTRA1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HTRA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HTRA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HTRA1. You can calculate the concentration of HTRA1 in the samples by comparing the RLU value of the samples to the standard curve.

225000652

AAI72536.1

51,287 Da

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]

HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family. Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease. Chromosomal Location of Human Ortholog: 10q26.3. Cellular Component: extracellular matrix; extracellular space; cytosol. Molecular Function: insulin-like growth factor binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: regulation of cell growth; negative regulation of defense response to virus; proteolysis; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of epithelial cell proliferation; placenta development; negative regulation of BMP signaling pathway. Disease: Macular Degeneration, Age-related, 7; Cerebral Autosomal Recessive Arteriopathy With Subcortical Infarcts And Leukoencephalopathy

48-Strip-Wells

450 USD

96-Strip-Wells

535

5x96-Strip-Wells

2255

10x96-Strip-Wells

3975