Human alpha1-AT (Alpha 1-Antitrypsin) CLIA Kit | MyBioSource MBS2530416 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

other

product name :

Human alpha1-AT (Alpha 1-Antitrypsin) CLIA Kit

catalog :

MBS2530416

quantity :

48-Strip-Wells

price :

450 USD

product information

catalog number :

MBS2530416

products type :

CLIA Kit

products full name :

Human alpha1-AT (Alpha 1-Antitrypsin) CLIA Kit

products short name :

[alpha1-AT (Alpha 1-Antitrypsin)]

products name syn :

[SERPINA1; A1A; A1AT; AAT; PI; PI1; PRO2275; Human a1-AT (Alpha 1-Antitrypsin) CLIA Kit; Human a1-AT]

other names :

[alpha-1-antitrypsin; Alpha-1-antitrypsin; alpha-1-antitrypsin; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1]

products gene name :

[a1-AT]

other gene names :

[SERPINA1; SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT; AAT; PI; SPAAT]

uniprot entry name :

A1AT_HUMAN

reactivity :

Human

sequence length :

418

specificity :

This kit recognizes natural and recombinant alpha1-AT. No significant cross-reactivity or interference between alpha1-AT and analogues was observed.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

products description :

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of alpha1-AT concentrations in serum, plasma and other biological fluids. Principle of the Assay This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to alpha1-AT. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for alpha1-AT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain alpha1-AT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of alpha1-AT. You can calculate the concentration of alpha1-AT in the samples by comparing the RLU value of the samples to the standard curve.

ncbi gi num :

189163542

ncbi acc num :

NP_001121179.1

ncbi gb acc num :

NM_001127707.1

ncbi mol weight :

34,755 Da

ncbi pathways :

Asparagine N-linked Glycosylation Pathway (1268714); COPII (Coat Protein 2) Mediated Vesicle Transport Pathway (1268727); Cargo Concentration In The ER Pathway (1309087); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); ER To Golgi Anterograde Transport Pathway (1268726); FOXA1 Transcription Factor Network Pathway (137979); Hemostasis Pathway (1269340); Membrane Trafficking Pathway (1269877)

ncbi summary :

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Inhibitor; Secreted. Chromosomal Location of Human Ortholog: 14q32.1. Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; ER to Golgi transport vesicle; ER-Golgi intermediate compartment membrane; extracellular region; extracellular space; Golgi apparatus; Golgi membrane; proteinaceous extracellular matrix. Molecular Function: glycoprotein binding; identical protein binding; protease binding; protein binding; serine-type endopeptidase inhibitor activity. Biological Process: acute-phase response; blood coagulation; cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; platelet activation; platelet degranulation; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine. Disease: Alpha-1-antitrypsin Deficiency; Pulmonary Disease, Chronic Obstructive

size1 :

48-Strip-Wells

price1 :

450 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2255

size4 :

10x96-Strip-Wells

price4 :

3975