antibody

PARK7 Antibody

MBS2530306

0.06 mL

185 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Antibody

PARK7 Antibody

PARK7

PARK7; DJ-1; DJ1; FLJ27376; FLJ34360; FLJ92274

protein DJ-1; Protein DJ-1; protein DJ-1; Parkinson disease (autosomal recessive, early onset) 7; epididymis secretory sperm binding protein Li 67p; oncogene DJ1; parkinson protein 7; Oncogene DJ1; Parkinson disease protein 7

PARK7

PARK7; PARK7; DJ1; DJ-1; HEL-S-67p

PARK7_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

189

Antigen affinity purification

1mg/mL

Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

WB: 1:500 - 1:2000. IHC: 1:50- 1:200. ICC: 1:20- 1:100. IF: 1:50- 1:200. IP: 1:20- 1:100

Background/Introduction: Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in alpha-synuclein, Parkin, and DJ-1 are linked to PD (1). alpha-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).

183227678

NP_001116849.1

NM_001123377.1

20kDa

Alpha-synuclein Signaling Pathway (137913); Androgen Receptor Signaling Pathway (198806); Parkinson's Disease Pathway (83098); Parkinsons Disease Pathway (705377); Synaptic Vesicle Pathway (672457)

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration. Protein type: Tumor suppressor; EC 3.4.-.-; Nuclear receptor co-regulator; Transcription regulation. Chromosomal Location of Human Ortholog: 1p36.23. Cellular Component: PML body; mitochondrion; axon; mitochondrial matrix; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; mitochondrial respiratory chain complex I; chromatin; cytosol; lipid raft. Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; mercury ion binding; transcription coactivator activity; transcription factor binding; mRNA binding; peptidase activity; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding. Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; regulation of mitochondrial membrane potential; negative regulation of protein amino acid phosphorylation; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of peptidyl-serine phosphorylation; negative regulation of protein sumoylation; methylglyoxal catabolic process to D-lactate; positive regulation of protein kinase B signaling cascade; membrane depolarization; detoxification of mercury ion; regulation of inflammatory response; Ras protein signal transduction; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; lactate biosynthetic process; negative regulation of protein kinase activity; autophagy; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of apoptosis. Disease: Parkinson Disease 7, Autosomal Recessive Early-onset

0.06 mL

185 USD

0.12 mL

255

0.2 mL

400