antibody

HLA-DPA1 Antibody

MBS2529717

0.06 mL

195 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

HLA-DPA1 Antibody

HLA-DPA1

DP(W3), DP (W4), HLA DP1A, HLA DPA1, HLA SB alpha chain, HLADP, HLADPA1

HLA class II histocompatibility antigen, DP alpha 1 chain; HLA class II histocompatibility antigen, DP alpha 1 chain; HLA class II histocompatibility antigen, DP alpha 1 chain; HLA-SB alpha chain; MHC class II DP3-alpha; MHC class II HLA-DPA1 antigen; MHC class II antigen; Primed lymphocyte test-1; major histocompatibility complex, class II, DP alpha 1; DP(W3); DP(W4); HLA-SB alpha chain; MHC class II DP3-alpha; MHC class II DPA1

HLA-DPA1

HLA-DPA1; HLA-DPA1; PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A; HLA-DP1A; HLASB

DPA1_HUMAN

Polyclonal

IgG

Rabbit

Human

260

Antigen affinity purification

Store at -20 degree C. Avoid freeze / thaw cycles

ELISA (EIA), Western Blot (WB)

WB: 1:500-1:5000

Immunogen: Fusion protein of HLA-DPA1. Calculated Molecular Weight: 60aa; 29kd. Observed Molecular Weight: 33kDa

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

335883187

NP_001229454.1

NM_001242525.1

29,381 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]

HLA-DPA1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; cell surface; integral to plasma membrane; lysosomal membrane; plasma membrane; endosome membrane; trans-Golgi network membrane; MHC class II protein complex. Molecular Function: MHC class II receptor activity; peptide antigen binding. Biological Process: positive regulation of interferon-gamma production; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; immune response; positive regulation of T cell activation; T cell receptor signaling pathway

0.06 mL

195 USD

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270

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