EIF2AK1 Antibody | MyBioSource MBS2526743 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EIF2AK1 Antibody

catalog :

MBS2526743

quantity :

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price :

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clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

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image

image 1 :

Western Blot of Hela cells using EIF2AK1 Polyclonal Antibody at dilution of 1:500

image 2 :

Immunofluorescent analysis of HepG2 cells, using EIF2AKl antibody at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

product information

catalog number :

MBS2526743

products type :

Antibody

products full name :

EIF2AK1 Antibody

products short name :

[EIF2AK1]

products name syn :

[EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369]

other names :

[Eukaryotic translation initiation factor 2-alpha kinase 1; Eukaryotic translation initiation factor 2-alpha kinase 1; eukaryotic translation initiation factor 2-alpha kinase 1; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; heme-controlled repressor; heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; hemin-sensitive initiation factor 2-alpha kinase; eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase]

products gene name :

[EIF2AK1]

other gene names :

[EIF2AK1; EIF2AK1; HCR; HRI; HRI; KIAA1369; HCR]

uniprot entry name :

E2AK1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

630

purity :

Antigen affinity purification

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

app notes :

WB: 1:500-1:3000. IF: 1:10-1:100

image1 heading :

Western Blot

image2 heading :

Immunofluorescent

other info1 :

Immunogen: Fusion protein of EIF2AK1. Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

products description :

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2aJGTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions.

ncbi gi num :

32172458

ncbi acc num :

Q9BQI3

ncbi mol weight :

Calculated Molecular Weight: 630aa; 71kDa. Observed Molecular Weight: 71kd

ncbi pathways :

Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217173); Influenza A Pathway (217150); Measles Pathway (213306); Measles Pathway (213277)

ncbi summary :

The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

HRI: a protein kinase of the PEK family. Phosphorylates the alpha subunit of eukaryotic initiation factor 2 in response to various stress conditions, inhibiting protein synthesis at the level of initiation. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity. Requires association with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosporylation. Two alternatively spliced isoforms have been described. Protein type: Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Translation; Other group; PEK family. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: cytoplasm. Molecular Function: protein homodimerization activity; heme binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity. Biological Process: response to external stimulus; negative regulation of cell proliferation; negative regulation of translational initiation by iron; protoporphyrinogen IX metabolic process; protein amino acid autophosphorylation; response to stress; negative regulation of hemoglobin biosynthetic process

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