FZR1 Antibody | MyBioSource MBS2525824 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FZR1 Antibody

catalog :

MBS2525824

quantity :

0.06 mL

price :

195 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of HeLa cells using FZR1 Polyclonal Antibody were subjected to SDS PAGE followed byat dilution of 1:1000.

image 2 :

Immunohistochemistry of paraffin embedded Human Liver Tissue slide using FZR1 Polyclonal Antibody at dilution od 1:200

product information

catalog number :

MBS2525824

products type :

Antibody

products full name :

FZR1 Antibody

products short name :

[FZR1]

products name syn :

[CDC20 like protein 1, CDC20C, CDH1, Cdh1/Hct1homolog, Fizzy related protein homolog, FYR, FZR,FZR1, FZR2, HCDH, HCDH1, KIAA1242]

other names :

[Fzr1; Fizzy-related protein homolog; fizzy-related protein homolog; CDC20-like 1b; CDC20-like protein 1; cdh1/Hct1 homolog; fizzy/cell division cycle 20 related 1 (Drosophila); CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1]

products gene name :

[FZR1]

other gene names :

[FZR1; FZR1; FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C; CDH1; FYR; FZR; KIAA1242; Fzr; hCDH1]

uniprot entry name :

FZR_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

496

purity :

Antigen affinity purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles

tested application :

WB, IF , ELISA

app notes :

WB 1:500-1:2000. IF 1:50-1:500. IHC 1:50-1:500

image1 heading :

Western Blot(WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Fusion protein of FZR1. Calculated MW: 493aa; 55kd. Observed MW: 55kd

other info2 :

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19398 / sc-19399

products description :

FZR1 gene, also named as FYR, FZR and KIAA1242, has beenpreviously shown to be mutated in lobular breast carcinomas. Directevidence of FZR1 mutations triggering tumorigenesis has beeninvestigated. FZR1 is a key regulator of ligase activity of the anaphasepromoting complex/cyclosome (APC/C) which is playing vital role s incell cycle progression. There are 7 WD-repeat domains in FZR1. Uponphosphorylation and dephosphorylation, it interacts with othercomponents to modulate cell mitosis. This gene is different to E-cadherin(CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54kDa and 45 kDa isoforms.

ncbi gi num :

6463679

ncbi acc num :

BAA86954.1

ncbi pathways :

APC/C Complex Pathway (413481); APC/C Complex Pathway (468395); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Aurora A Signaling Pathway (137925); Autodegradation Of Cdh1 By Cdh1:APC/C Pathway (105836); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765)

uniprot summary :

FZR1: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1- mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Belongs to the WD repeat CDC20/Fizzy family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: nucleoplasm; nuclear membrane; anaphase-promoting complex; cytosol. Molecular Function: protein binding. Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; positive regulation of protein catabolic process; regulation of meiosis; mitotic cell cycle; G2/M transition DNA damage checkpoint; DNA repair

size1 :

0.06 mL

price1 :

195 USD

size2 :

0.12 mL

price2 :

270

size3 :

0.2 mL

price3 :

435

more info or order :

MyBioSource product webpage