TBC1D4 Polyclonal Antibody | MyBioSource MBS2524412 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TBC1D4 Polyclonal Antibody

catalog :

MBS2524412

quantity :

0.02 mL

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of Hela and hepg2 cell using TBC1D4 Polyclonal Antibody at dilution of 1:320

image 2 :

Immunohistochemistry of paraffin-embedded Human breast cancer using TBC1D4 Polyclonal Antibody at dilution of 1:40

product information

catalog number :

MBS2524412

products type :

Antibody

products full name :

TBC1D4 Polyclonal Antibody

products short name :

[TBC1D4]

products name syn :

[AS160]

other names :

[TBC1 domain family member 4 isoform 2; TBC1 domain family member 4; TBC1 domain family member 4; Acrg embryonic lethality minimal region ortholog; TBC (Tre-2, BUB2, CDC16) domain-containing protein; akt substrate of 160 kDa; TBC1 domain family, member 4; Akt substrate of 160 kDa; AS160]

products gene name :

[TBC1D4]

other gene names :

[TBC1D4; TBC1D4; AS160; NIDDM5; AS160; KIAA0603; AS160]

uniprot entry name :

TBCD4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

1290

purity :

Antigen affinity purification

concentration :

2.6mg/mL

storage stability :

Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500-1:2000. IHC: 1:30-1:150

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

products description :

Background/Introduction: TBC1 domain family member 4 (TBC1D4), also designated AS160, can be insulin- and/or AKT1- induced. Insulin-stimulated phosphorylation is required for GLUT4 translocation. TBC1D4 may play a role as a GTPase activating protein for proteins in the Rab family. It is expressed primarily in skeletal muscle and heart, as well as spleen, lymph node and leukocytes. Defects in the TBC1D4 gene may cause atopic dermatitis (AD), sometimes referred to as eczema, an atopic chronic skin disease. The skin of affected individuals reacts to irritants or allergens and becomes red, flaky and itchy. The skin is also more vulnerable to inflammations, and symptoms can grow or disappear over time.

ncbi gi num :

557786188

ncbi acc num :

NP_001273587.1

ncbi gb acc num :

NM_001286658.1

ncbi mol weight :

60,095 Da

ncbi pathways :

Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Insulin Signaling Pathway (198845); Insulin-mediated Glucose Transport Pathway (137981); Membrane Trafficking Pathway (106160); Thyroid Hormone Signaling Pathway (946598); Translocation Of GLUT4 To The Plasma Membrane Pathway (685554)

ncbi summary :

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

uniprot summary :

AS160: a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue. Protein type: GAPs; GAPs, Rab. Chromosomal Location of Human Ortholog: 13q22.2. Cellular Component: cytoplasmic vesicle membrane; cytoplasm. Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus. Disease: Diabetes Mellitus, Noninsulin-dependent, 5

size1 :

0.02 mL

price1 :

110 USD

size2 :

0.06 mL

price2 :

150

size3 :

0.12 mL

price3 :

225

size4 :

0.2 mL

price4 :

360

more info or order :

MyBioSource product webpage