EPS8 Antibody | MyBioSource MBS2523843 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EPS8 Antibody

catalog :

MBS2523843

quantity :

0.06 mL

price :

195 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

human brain tissue were subjected to SDS-PAGE followed by western blot with EPS8 Polyclonal Antibody at dilution of 1:500

image 2 :

Immunofluorescent analysis of A375 ells using EPS8 Antibody at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti- Rabbit IgG(H+L)

image 3 :

Immunohistochemistry of paraffinembedded human breast cancer tissue slide using EPS8 Polyclonal Antibody at dilution of 1:100 (under 40x lens).

product information

catalog number :

MBS2523843

products type :

Antibody

products full name :

EPS8 Antibody

products short name :

[EPS8]

other names :

[epidermal growth factor receptor kinase substrate 8; Epidermal growth factor receptor kinase substrate 8; epidermal growth factor receptor kinase substrate 8; epidermal growth factor receptor pathway substrate 8]

products gene name :

[EPS8]

other gene names :

[EPS8; EPS8; DFNB102]

uniprot entry name :

EPS8_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

822

purity :

Antigen affinity purification

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:200-1:2000. IHC: 1:20-1:200. IF: 1:10-1:100

image1 heading :

SDS-PAGE

image2 heading :

Immunofluorescent

image3 heading :

Immunohistochemistry

other info1 :

Immunogen: Fusion protein of EPS8. Calculated Molecular Weight: 822aa; 92kd. Observed Molecular Weight: 97kd

other info2 :

Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30778 / sc-30776 / sc-30777 / sc-30778 / sc-30776 / sc-30777 / sc-1841

products description :

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.

ncbi gi num :

56682953

ncbi acc num :

NP_004438.3

ncbi gb acc num :

NM_004447.5

uniprot acc num :

Q12929

ncbi pathways :

CDC42 Signaling Events Pathway (137994); EGFR1 Signaling Pathway (198782); ErbB1 Downstream Signaling Pathway (138057); Regulation Of RAC1 Activity Pathway (169346)

ncbi summary :

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

EPS8: an adaptor protein that is an epidermal growth factor receptor (EGFR) substrate. Enhances EGF-dependent mitogenic signals. Member of a family of actin barbing and capping proteins. Promotes filopodial protrusions. Complexes containing Eps8/Abi1/PI3K/Sos-1 mediate Rac activation following EGFR signaling. Involved in endocytosis of EGFR by binding to the Rab5-specific GTPase activating protein USP6N. Binds to IRSp53, which binds small the small GTPases Rac and Cdc42, and which regulates actin dynamics. Involved in the actin-remodeling activity in cultured cerebellar neurons. Regulates the actin-remodeling activities of NMDA and ethanol in neurons. Eps8 knockout mice show behavioral resistance to ethanol. Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 12p12.3. Cellular Component: stereocilium; growth cone; postsynaptic density; cell cortex; cell junction; vesicle; N-methyl-D-aspartate selective glutamate receptor complex. Molecular Function: protein binding; SH3/SH2 adaptor activity; Rac GTPase binding; actin binding. Biological Process: actin crosslink formation; epidermal growth factor receptor signaling pathway; actin filament bundle formation; positive regulation of signal transduction; adult locomotory behavior; Rac protein signal transduction; behavioral response to ethanol; signal transduction; regulation of cell shape; cell proliferation; actin cytoskeleton reorganization; regulation of actin filament length; barbed-end actin filament capping; exit from mitosis. Disease: Deafness, Autosomal Recessive 102

size1 :

0.06 mL

price1 :

195 USD

size2 :

0.12 mL

price2 :

270

size3 :

0.2 mL

price3 :

435

more info or order :

MyBioSource product webpage