Human ARG2 (Arginase II) ELISA Kit | MyBioSource MBS2516353 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ARG2 (Arginase II) ELISA Kit

catalog :

MBS2516353

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2516353

products type :

ELISA Kit

products full name :

Human ARG2 (Arginase II) ELISA Kit

products short name :

ARG2

other names :

ARG2; Arginase-2, mitochondrial; arginase-2, mitochondrial; L-arginine amidinohydrolase; L-arginine ureahydrolase; arginase, type II; kidney arginase; kidney-type arginase; non-hepatic arginase; nonhepatic arginase; type II arginase; arginase 2; Kidney-type arginase; Non-hepatic arginase; Type II arginase

products gene name :

ARG2

other gene names :

ARG2; ARG2

uniprot entry name :

ARGI2_HUMAN

reactivity :

Human

sequence length :

354

specificity :

This kit recognizes natural and recombinant Human ARG2. No significant cross-reactivity or interference between Human ARG2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: 1.875ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ARG2 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ARG2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ARG2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ARG2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ARG2. You can calculate the concentration of ARG2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

49168584

ncbi acc num :

CAG38787.1

uniprot acc num :

P78540

ncbi mol weight :

38,578 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (413403)

ncbi summary :

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

ARG2: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. Belongs to the arginase family. Protein type: EC 3.5.3.1; Hydrolase; Mitochondrial; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 14q24.1. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: metal ion binding; arginase activity. Biological Process: striated muscle contraction; arginine metabolic process; ureteric bud development; nitric oxide biosynthetic process; urea cycle

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage