Mouse NT-3 (Neurotrophin-3) ELISA Kit | MyBioSource MBS2516352 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse NT-3 (Neurotrophin-3) ELISA Kit

catalog :

MBS2516352

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2516352

products type :

ELISA Kit

products full name :

Mouse NT-3 (Neurotrophin-3) ELISA Kit

products short name :

[NT-3]

other names :

[Neurotrophin-3; Neurotrophin-3; neurotrophin-3; nerve growth factor 2; neurotrophic factor; neurotrophin 3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor]

products gene name :

[NT-3]

other gene names :

[NTF3; NTF3; NT3; HDNF; NGF2; NT-3; NGF-2; NT-3; NGF-2]

uniprot entry name :

NTF3_HUMAN

reactivity :

Mouse

sequence length :

257

specificity :

This kit recognizes natural and recombinantMouseNT-3. No significant cross-reactivity or interference between MouseNT-3 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseNT-3concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NT-3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NT-3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NT-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NT-3.You can calculate the concentration of NT-3in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

128581

ncbi acc num :

P20783.1

ncbi mol weight :

30,800 Da

ncbi pathways :

BDNF Signaling Pathway (712093); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Neurotrophic Factor-mediated Trk Receptor Signaling Pathway (137977); Neurotrophin Signaling Pathway (101143); Neurotrophin Signaling Pathway (100064); Trk Receptor Signaling Mediated By The MAPK Pathway (138044); P75(NTR)-mediated Signaling Pathway (138019)

ncbi summary :

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]

uniprot summary :

NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region. Molecular Function: neurotrophin p75 receptor binding; growth factor activity; nerve growth factor binding; receptor binding; chemoattractant activity. Biological Process: myelination; axon guidance; epidermis development; mechanoreceptor differentiation; glial cell fate determination; regulation of neuron differentiation; activation of MAPK activity; positive regulation of receptor internalization; positive regulation of glial cell differentiation; signal transduction; enteric nervous system development; induction of positive chemotaxis; cell-cell signaling; positive chemotaxis; positive regulation of cell proliferation; negative regulation of neuron apoptosis; regulation of synaptic transmission; nervous system development; negative regulation of peptidyl-tyrosine phosphorylation; activation of protein kinase B; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; neuromuscular synaptic transmission; positive regulation of transcription from RNA polymerase II promoter; nerve development; smooth muscle cell differentiation; brain development; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; positive regulation of cell migration

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590