ELISA/assay

Rat PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

MBS2516345

24-Strip-Wells

215 USD

ELISA Kit

Rat PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

[PACAP]

[PACAP; Pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP; adenylate cyclase activating polypeptide 1 (pituitary); PRP-48]

[PACAP]

[ADCYAP1; ADCYAP1; PACAP; PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38]

PACA_HUMAN

Rat

176

This kit recognizes natural and recombinantRatPACAP. No significant cross-reactivity or interference between RatPACAP and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 7.813-500pg/mL. Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatPACAPconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PACAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PACAPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PACAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PACAP.You can calculate the concentration of PACAPin the samples by comparing the OD of the samples to the standard curve.

1132550

CAA42962.1

P18509

18,835 Da

Activation Of TRKA Receptors Pathway (106456); Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Insulin Secretion Pathway (777534); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 18p11. Cellular Component: extracellular space; extracellular region; terminal button. Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor signaling protein activity; pituitary adenylate cyclase-activating peptide receptor binding; pituitary adenylate cyclase activating polypeptide activity; receptor binding. Biological Process: behavioral fear response; nerve growth factor receptor signaling pathway; negative regulation of potassium ion transport; female pregnancy; positive regulation of vasodilation; sensory perception of pain; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell cycle; histamine secretion; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; regulation of protein localization; cell-cell signaling; ovarian follicle development; neuropeptide signaling pathway; positive regulation of cell proliferation; neurite development; negative regulation of acute inflammatory response to antigenic stimulus; adenylate cyclase activation; positive regulation of interleukin-6 production; positive regulation of growth hormone secretion; ATP metabolic process; response to starvation; response to ethanol; regulation of postsynaptic membrane potential; pituitary gland development; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of acute inflammatory response to non-antigenic stimulus; regulation of G-protein coupled receptor protein signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590