Human CD34 (Cluster Of Differentiation 34) ELISA Kit | MyBioSource MBS2516296 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CD34 (Cluster Of Differentiation 34) ELISA Kit

catalog :

MBS2516296

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2516296

products type :

ELISA Kit

products full name :

Human CD34 (Cluster Of Differentiation 34) ELISA Kit

products short name :

[CD34]

other names :

[CD34; Hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell antigen CD34; CD34 antigen; CD34 molecule; CD_antigen: CD34]

products gene name :

[CD34]

other gene names :

[CD34; CD34]

uniprot entry name :

CD34_HUMAN

reactivity :

Human

sequence length :

328

specificity :

This kit recognizes Human CD19 in samples. No Significant cross-reactivity or interference between Human CD19 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20 ng/mL. Sensitivity: 0.19 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CD19 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CD19 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CD19 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD19. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD19, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CD19. You can calculate the concentration of Human CD19 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

264769

ncbi acc num :

AAB25223.1

ncbi mol weight :

35,011 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); C-MYB Transcription Factor Network Pathway (138073); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

ncbi summary :

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: intercellular bridge; lysosome; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding. Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; positive regulation of angiogenesis; cell-cell adhesion; endothelial cell proliferation; regulation of blood pressure; tissue homeostasis; hemopoiesis; leukocyte migration

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590