ELISA/assay

Human PIBF (Progesterone-induced-blocking factor) ELISA Kit

MBS2516289

24-Strip-Wells

215 USD

ELISA Kit

Human PIBF (Progesterone-induced-blocking factor) ELISA Kit

[PIBF]

[progesterone-induced-blocking factor 1; Progesterone-induced-blocking factor 1; progesterone-induced-blocking factor 1; progesterone-induced blocking factor 1; progesterone immunomodulatory binding factor 1]

[PIBF]

[PIBF1; PIBF1; PIBF; CEP90; C13orf24; C13orf24; PIBF]

PIBF1_HUMAN

Human

757

This kit recognizes natural and recombinantHumanPIBF. No significant cross-reactivity or interference between HumanPIBF and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanPIBFconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PIBF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PIBFand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PIBF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PIBF.You can calculate the concentration of PIBFin the samples by comparing the OD of the samples to the standard curve.

55769583

NP_006337.2

NM_006346.2

12,016 Da

PIBF1: Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. 3 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 13q22.1. Cellular Component: centrosome. Molecular Function: protein binding

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590