ELISA/assay

Human PROM1(Prominin-1)ELISA Kit

MBS2516226

48-Strip-Wells

410 USD

ELISA Kit

Human PROM1(Prominin-1)ELISA Kit

PROM1

PROM1 protein; Prominin-1; prominin-1; antigen AC133; hProminin; hematopoietic stem cell antigen; prominin-like protein 1; prominin 1; Antigen AC133; Prominin-like protein 1; CD_antigen: CD133

PROM1

PROM1; PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; PROML1

PROM1_HUMAN

Human

856

This kit recognizes natural and recombinant Human PROM1. No significant cross-reactivity or interference between Human PROM1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PROM1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PROM1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PROM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PROM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PROM1. You can calculate the concentration of PROM1 in the samples by comparing the OD of the samples to the standard curve.

15082356

AAH12089.1

94,605 Da

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 4p15.32. Cellular Component: stereocilium; extracellular space; cell surface; photoreceptor outer segment; microvillus membrane; integral to plasma membrane; endoplasmic reticulum; apical plasma membrane; plasma membrane; ER-Golgi intermediate compartment; brush border; vesicle. Molecular Function: actinin binding; protein binding; cadherin binding. Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye. Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12

48-Strip-Wells

410 USD

96-Strip-Wells

490