catalog number :
MBS2516225
products type :
ELISA Kit
products full name :
Human MMP23B (Matrix Metalloproteinase 23B) ELISA Kit
products short name :
[MMP23B]
other names :
[Matrix metalloproteinase-23; Matrix metalloproteinase-23; matrix metalloproteinase-23; MMP-21; MMP-22; MMP-23; femalysin; matrix metalloproteinase 22; matrix metalloproteinase 23B; matrix metalloproteinase in the female reproductive tract; matrix metalloproteinase-21; matrix metalloproteinase-22; matrix metallopeptidase 23B; Femalysin; MIFR-1; Matrix metalloproteinase-21; MMP-21; Matrix metalloproteinase-22; MMP-22]
products gene name :
[MMP23B]
other gene names :
[MMP23B; MMP23A; MIFR; MMP22; MIFR-1; MMP23A; MMP21; MMP-23; MMP-21; MMP-22]
uniprot entry name :
MMP23_HUMAN
specificity :
This kit recognizes Human MeCP2 in samples. No significant cross-reactivity or interference between Human MeCP2 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MeCP2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MeCP2 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MeCP2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MeCP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MeCP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MeCP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MeCP2. You can calculate the concentration of Human MeCP2 in the samples by comparing the OD of the samples to the standard curve.
ncbi mol weight :
46,305 Da
ncbi pathways :
Matrix Metalloproteinases Pathway (198900)
ncbi summary :
This gene (MMP23B) encodes a member of the matrix metalloproteinase (MMP) family, and it is part of a duplicated region of chromosome 1p36.3. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene belongs to the more telomeric copy of the duplicated region. [provided by RefSeq, Jul 2008]
uniprot summary :
MMP23B: Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum. Belongs to the peptidase M10A family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 3.4.24.-; Protease. Chromosomal Location of Human Ortholog: 1p36.3. Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum membrane; integral to membrane; intracellular. Molecular Function: zinc ion binding; metallopeptidase activity; metalloendopeptidase activity. Biological Process: proteolysis; reproduction
size5 :
10x96-Strip-Wells