ELISA/assay

Human ITGB3 (Integrin Beta 3) ELISA Kit

MBS2516199

24-Strip-Wells

215 USD

ELISA Kit

Human ITGB3 (Integrin Beta 3) ELISA Kit

[ITGB3]

[integrin beta-3; Integrin beta-3; integrin beta-3; platelet membrane glycoprotein IIIa; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa; GPIIIa; CD_antigen: CD61]

[ITGB3]

[ITGB3; ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; GP3A; GPIIIa]

ITB3_HUMAN

Human

788

This kit recognizes natural and some recombinant Human ITGB3. No significant crossreactivity or interference between Human ITGB3 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human ITGB3 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human ITGB3. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human ITGB3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human ITGB3, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ITGB3. The concentration of Human ITGB3 in samples can be calculated by comparing the OD of the samples with the standard curve.

47078292

NP_000203.2

NM_000212.2

86,694 Da

Arf6 Signaling Events Pathway (138034); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); Cell Surface Interactions At The Vascular Wall Pathway (106062); Developmental Biology Pathway (477129); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); ECM Proteoglycans Pathway (833812)

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 17q21.32. Cellular Component: filopodium membrane; platelet alpha granule membrane; focal adhesion; cell surface; integral to plasma membrane; microvillus membrane; plasma membrane; melanosome; nucleus; integrin complex; receptor complex. Molecular Function: identical protein binding; protein binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; platelet-derived growth factor receptor binding; protein disulfide isomerase activity; receptor activity; cell adhesion molecule binding. Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; protein folding; wound healing; cell-matrix adhesion; smooth muscle cell migration; mesodermal cell differentiation; negative chemotaxis; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell adhesion; cell growth; integrin-mediated signaling pathway; platelet activation; cell migration; regulation of bone resorption; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; tube development; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration. Disease: Myocardial Infarction, Susceptibility To; Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590