catalog number :
MBS2516191
products type :
ELISA Kit
products full name :
Canine IFN-alpha (Interferon Alpha) ELISA Kit
products short name :
[IFN-alpha]
other names :
[interferon alpha-1/13; Interferon alpha-1/13; interferon alpha-1/13; IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-D; interferon-alpha1; leIF D; interferon, alpha 1; Interferon alpha-D; LeIF D]
products gene name :
[IFN-alpha]
other gene names :
[IFNA1; IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD; IFN-alpha-1/13; LeIF D]
uniprot entry name :
IFNA1_HUMAN
specificity :
This kit recognizes Canine Cys-C in samples. No significant cross-reactivity or interference between Canine Cys-C and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level CanineCys-C were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision(Precision between assays): 3 samples with low, midrange and high level CanineCys-C were testedon 3 different plates, 20replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Canine Cys-C concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Canine Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Canine Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Canine Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Canine Cys-C. You can calculate the concentration of Canine Cys-C in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_076918.1
ncbi gb acc num :
NM_024013.2
ncbi mol weight :
21,725 Da
ncbi pathways :
Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis B Pathway (694606)
ncbi summary :
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
uniprot summary :
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; blood coagulation; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein
size5 :
10x96-Strip-Wells