ELISA/assay

Human suPAR(soluble urokinase-type plasminogen activator receptor) ELISA Kit

MBS2516189

48-Strip-Wells

410 USD

ELISA Kit

Human suPAR(soluble urokinase-type plasminogen activator receptor) ELISA Kit

suPAR

soluble urokinase plasminogen activator receptor; Urokinase plasminogen activator surface receptor; urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor; plasminogen activator, urokinase receptor; Monocyte activation antigen Mo3; CD_antigen: CD87

suPAR

PLAUR; PLAUR; CD87; UPAR; URKR; U-PAR; MO3; UPAR; U-PAR; uPAR

UPAR_HUMAN

Human

301

This kit recognizes natural and recombinant Human suPAR. No significant cross-reactivity or interference between Human suPAR and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.094ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human suPAR concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to suPAR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for suPAR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain suPAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of suPAR. You can calculate the concentration of suPAR in the samples by comparing the OD of the samples to the standard curve.

13641309

AAK31795.1

32,016 Da

Arf6 Downstream Pathway (138038); Attachment Of GPI Anchor To UPAR Pathway (106239); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); FGF Signaling Pathway (137989); Hemostasis Pathway (106028); Metabolism Of Proteins Pathway (106230); Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway (106236)

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Receptor, misc. Chromosomal Location of Human Ortholog: 19q13. Cellular Component: endoplasmic reticulum membrane; focal adhesion; extrinsic to membrane; integral to plasma membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane. Molecular Function: protein domain specific binding; protein binding; enzyme binding; U-plasminogen activator receptor activity; receptor activity; receptor binding. Biological Process: fibrinolysis; cellular protein metabolic process; regulation of proteolysis; attachment of GPI anchor to protein; C-terminal protein lipidation; chemotaxis; post-translational protein modification; blood coagulation; signal transduction; cell motility

48-Strip-Wells

410 USD

96-Strip-Wells

490