catalog number :
MBS2516160
products type :
ELISA Kit
products full name :
T (Testosterone) ELISA Kit
products short name :
[Testosterone]
specificity :
This kit recognizes Sheepalpha1+AGPin samples. No significantcross-reactivity or interference between Sheepalpha1-AGP and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.813-500ng/mL. Sensitivity: 4.688ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Sheepalpha1-AGP were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Sheepalpha1-AGP were testedon 3 different plates, 20 replicates in eachplate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheepalpha1-AGP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has beenpre-coated with an antibody specific to Sheepalpha1-AGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Sheepalpha1-AGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Sheepalpha1-AGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Sheepalpha1-AGP. You can calculate the concentration of Sheepalpha1-AGP in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells