Mouse Klotho ELISA Kit | MyBioSource MBS2516159 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Klotho ELISA Kit

catalog :

MBS2516159

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2516159

products type :

ELISA Kit

products full name :

Mouse Klotho ELISA Kit

products short name :

[Klotho]

other names :

[klotho; Klotho; klotho; klotho]

products gene name :

[Klotho]

other gene names :

[KL; KL]

uniprot entry name :

KLOT_HUMAN

reactivity :

Mouse

sequence length :

1012

specificity :

This kit recognizes Mouse KISS1 in samples. No significant cross-reactivity or interference between Mouse KISS1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse KISS1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse KISS1 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse KISS1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse KISS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse KISS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse KISS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse KISS1. You can calculate the concentration of Mouse KISS1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

2618596

ncbi acc num :

BAA23382.1

ncbi mol weight :

62,135 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276)

ncbi summary :

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

uniprot summary :

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.2.1.31; Hydrolase; Membrane protein, integral. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane. Molecular Function: vitamin D binding; signal transducer activity; beta-glucuronidase activity; beta-glucosidase activity; fibroblast growth factor binding; hormone activity; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; calcium ion homeostasis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; insulin receptor signaling pathway; energy reserve metabolic process; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging. Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590